TRYPANOSOMA EVANSI AND NEOSPORA CANINUM AMONG WATER BUFFALOES (BUBALUS BUBALIS) IN THE PHILIPPINES

Authors

  • Claro Niegos Mingala Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines http://orcid.org/0000-0002-0435-2156
  • Jaypee A. Abenoja College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines
  • Christopher V. Rivera College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines
  • Michelle M. Balbin Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines
  • Virginia M. Venturina College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines
  • Marvin A. Villanueva Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines

DOI:

https://doi.org/10.5380/avs.v25i1.67943

Keywords:

Trypanosoma, Neospora, PCR, cELISA, water buffalo

Abstract

The study determined the positivity rate of Trypanosoma evansi and Neospora caninum antibodies in water buffaloes in the province of Nueva Ecija, Philippines using Polymerase Chain Reaction (PCR) for T. evansi and competitive Enzyme-linked Immunosorbent Assay (cELISA) for N. caninum antibodies . A total of 100 whole blood and 100 serum samples were collected to test for T. evansi and N. caninum , respectively. Rotat 1.2 VSG gene was target using PCR for T. evansi detection. Neospora caninum antibody detection was done from the serum samples using cELISA test kit.Results revealed that the positivity rate of T. evansi in Nueva Ecija was 11% (11/100). The positive animals identified were from the municipalities of Muñoz (4/16; 25%), Sta. Rosa (3/13; 23.08%) and Talugtug (4/16; 25%). The seropositive rate of Nueva Ecija for N. caninum. was 46% (46/100), seropositive animals were identified in Cabanatuan City, 57.14% (4/7); Science City of Muñoz, 43.14% (22/51); Sta. Rosa, 40% (4/10); Sto. Sunday, 50% (6/12); and Talugtug 50% (10/20). The seropositivity rate of N. caninum and the presence of T. evansi in Nueva Ecija may contribute to the cases of abortions in the province and further studies should be employed to confirm the association of these organisms to abortion cases on water buffaloes.

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Published

2020-03-31

How to Cite

Mingala, C. N., Abenoja, J. A., Rivera, C. V., Balbin, M. M., Venturina, V. M., & Villanueva, M. A. (2020). TRYPANOSOMA EVANSI AND NEOSPORA CANINUM AMONG WATER BUFFALOES (BUBALUS BUBALIS) IN THE PHILIPPINES. Archives of Veterinary Science, 25(1). https://doi.org/10.5380/avs.v25i1.67943

Issue

Vol. 25 No. 1 (2020)

Section

Veterinary Parasitology

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