PHYTOHAEMAGGLUTININ’S EFFECT ON NEOSPORA CANINUM ANTIGEN PRODUCTION

Authors

  • Mariana Kikuti UNESP
  • Virgínia Bodelão Richini-Pereira UNESP
  • Rodrigo Costa da Silva UNESP
  • Helio Langoni UNESP

DOI:

https://doi.org/10.5380/avs.v18i1.27291

Keywords:

cultura celular, gerbil, quantificação

Abstract

The parasite Neospora caninum affects mainly cattle and dogs. This study aimed to evaluate the effect of phytohaemagglutinin (PHE) in antigen production of N. caninum NC-1 strain in gerbils (Meriones unguiculatus) and in vitro. 20 gerbils were used, 10 inoculated intraperitoneally with 1 x 106 tachyzoites and 10 with 1 x 106 tachyzoites plus 300 µL/mL of PHE. 16 bottles of Vero cell culture were inoculated, 8 with 1.5 x 105 tachyzoites and 8 with 1.5 x 105 tachyzoites plus 30 µL/mL of PHE. Serology of gerbils was performed on day 0 and before euthanasia. Tachyzoites present in peritoneal fluid and cell culture bottles were quantified by Neubauer chamber and by real-time PCR (qPCR). PHE has not interfered in the production of tachyzoites of N. caninum in intraperitoneal inoculated gerbils and the effect of PHE in cell culture had a negative impact, considering the qPCR technique as the gold standard.

Author Biographies

Mariana Kikuti, UNESP

Núcleo de Pesquisas em Zoonoses, Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista “Júlio de Mesquita Filho” campus Botucatu 

Virgínia Bodelão Richini-Pereira, UNESP

Núcleo de Pesquisas em Zoonoses, Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista “Júlio de Mesquita Filho” campus Botucatu 

Rodrigo Costa da Silva, UNESP

Núcleo de Pesquisas em Zoonoses, Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista “Júlio de Mesquita Filho” campus Botucatu 

Helio Langoni, UNESP

Núcleo de Pesquisas em Zoonoses, Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista “Júlio de Mesquita Filho” campus Botucatu 

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Published

2013-02-20

How to Cite

Kikuti, M., Richini-Pereira, V. B., da Silva, R. C., & Langoni, H. (2013). PHYTOHAEMAGGLUTININ’S EFFECT ON NEOSPORA CANINUM ANTIGEN PRODUCTION. Archives of Veterinary Science, 18(1). https://doi.org/10.5380/avs.v18i1.27291

Issue

Vol. 18 No. 1 (2013)

Section

Scientific Notes

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