Neuro-protective Efficacy of Apigenin in Induced Oxidative Stress Adult Male Rats

Abstract

This study was conducted to evaluate the promising antioxidant efficacy of apigenin extracted from parsley seeds in glycosidic (aqueous extract) and aglyconic forms (acid hydrolysis and organic solvent extraction), comparing to synthetic antioxidant, (Butylated hydroxytolouene) to ameliorate neurobehavioral disorders in experimentally induced oxidative stress adult male rats. Oral administration of 0.75% H2O2 (exogenic oxidant as reactive oxygen species donar) in drinking water for eight weeks was used for oxidative stress induction. The recorded results showed that oxidative stress has caused significant (P< 0.05) alterations in the neurobehavior of experimental animals in comparison to the control. The animals manifested a significant decrease (P<0.05) in locomotor, and exploration (open field test), defect in vestibular functions (negative geotaxis test), degree of cognitive function, and neuromuscular coordination (rotarod test). Mild (non-significant) changes were recorded in autonomic nervous system activity (number of urination and defecation) in H2O2 treated group, and less or non-significant changes in the other groups. The daily administration of apigenin (150 mg/kg body weight) in glycosidic and aglycon forms protected neurons probably by reducing oxidative damage and attenuated significantly the neurobehavioral disorders and imposed its neuro-protective and sedative effects in comparison to butylated hydroxytoloune (25 mg/kg body weight) treated and control groups.