Differential expression of cytokines in the pressure and tension sides during the orthodontic movement

Abstract

The aim of this work was evaluate the pro and antinflamatory cytokines expression, collagen type-I, remodeling tecidual mediator (MMP-1) and its inhibitor (TIMP-1), osteoclastogenic factors (RANKL) and osteoblastogenics (CBFA-1), in the different sides under tension forces and pressure, in the orthodontic movement. Quantitative examinations of reaction in chain of polymerase was realized (RealTime-PCR), using mRNA proceeding from samples of periodontal tooth ligament submitted to orthodontic forces. Tooth that has orthodontic extraction indication was utilized, after application of forces in maxillary and no-movement tooth in the control group. The results showed that the side of pressure had more expression of TGF-beta, TNF-a, RANKL e MMP1, and less expression of COL-1 when compared with control group, while tension side showed more expression of TGF-beta, IL-10, COL-1 and CBFA-1. Comparing the both sides was observed more expression of IL-10, COL-1 and CBFA-1 in the tension side, and more expression of TNF-a, RANKL and MMP-1 in the pressure side. The results indicate that cytokines standard determines the nature of the tecidual reply to the orthodontic force, modifying and guiding the equilibry between reabsortion and neoformation of the bone.